Regulatory

Part:BBa_K239000:Design

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-20)

DegP promoter, activated by Phosporylated CpxR and SigmaE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. Only one SpeI restriction site had to be removed. The sequence starts 3 bp before the first CpxR binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Sequence modified to remove Spe1 restriction site.

References

Bury-Moné, Stéphanie, Yanoura Nomane, Nancie Reymond, Romain Barbet, Eric Jacquet, Sandrine Imbeaud, Annick Jacq, and Philippe Bouloc (2009) Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli. PLoS Genet. 2009 September; 5(9): e1000651. Published online 2009 September 18. doi: 10.1371/journal.pgen.1000651. PMCID: PMC2731931


Costanzo, A., and S.E. Ades. (2006) Growth Phase-Dependent Regulation of the Extracytoplasmic Stress Factor, _E, by Guanosine 3_,5_-Bispyrophosphate (ppGpp). JOURNAL OF BACTERIOLOGY, July 2006, p. 4627–4634


DiGiuseppe, P. A., and T. J. Silhavy. (2003) Signal detection and target gene induction by the CpxRA two-component system. J. Bacteriol. 185:2432– 2440.


Hayden JD, Ades SE (2008) The extracytoplasmic stress factor, sigmaE, is required to maintain cell envelope integrity in Escherichia coli. PLoS ONE 3: e1573. doi:10.1371/journal.pone.0001573.


Miyadai, H., Tanaka-Masuda, K., Matsuyama, S., and Tokuda, H. (2004) Effects of Lipoprotein Overproduction on the Induction of DegP (HtrA) Involved in Quality Control in the Escherichia coli Periplasm*. THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 279, No. 38, Issue of September 17, pp. 39807–39813, 2004


Price, N. L., and T. L. Raivio. (2009) Characterization of the Cpx Regulon in Escherichia coli Strain MC4100 JOURNAL OF BACTERIOLOGY, Mar. 2009, p. 1798–1815


Raivio, T. L., M. W. Laird, J. C. Joly, and T. J. Silhavy. (2000) Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response. Mol. Microbiol. 37:1186–1197.


Rhodius, V.A., Suh, W.C., Nonaka, G., West, J., and Gross, C.A. (2005) Conserved and variable functions of the sigma(E) stress response in related genomes. PLoS Biol 4: e2.


Ruiz, N., and Silhavy, T.J (2005) Sensing external stress: watchdogs of the Escherichia coli cell envelope. Current Opinion in Microbiology 2005, 8:122–126


Snyder, Larry, and Wendy Champness. (2007) Molecular genetics of bacteria, 3rd ed. ASM Press. Washington, DC.


Wulf, P.D., McGuire, A.M., Liu, X., and Lin, E.C.C. (2002) Genome-wide profiling of promoter recognition by the twocomponent response regulator CpxR-P in Escherichia coli. J Biol Chem 277: 26652–26661.